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Sunday, September 15, 2013

Fruit flies

Drosophila melanogaster A Lab Write up utilization: In this experiment, Drosophila melanogaster (fruit fly) were used to observe genetic crosses. information was placid from an unknown F1 contemporaries, and the results of the F2 geneproportionn were used to determine the genotypes and phenotypes of both the F1 and agnate gene symmetryns. The F1 generation of A flies was concluded to be heterozygous for eye color bolshy dominant over recessive allele allele sepia- and the p atomic number 18ntal generation was determined to be homozygous. The phenotype ratio of the F2 generation was 3:1 with a 75% calamity of red eyes and 25% chance of sepia eyes. The genotype ratio was concluded to be 1:2:1: 25% pure-breeding for the dominant trait, 50% hybrid for the two traits, and 25% pure-breeding for the recessive trait. Procedure: 1.Make a habitat for the flies by obtaining an empty vial, foam, and plastic net. Mix a scoop of the exacting food medium with a scoop of water. 2.Obtain a Petri cup of tea of A flies and immobilize the flies by placing the Petri looker in the freezer for cristal legal proceeding. 3.Determine the sexual urge of the flies (sexing) by looking at the following characteristics finished and through a stereoscope. Males are usually smaller than females. Males take over dark, candid abdomens, and females remove lighter, pointed abdomens.
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Only the males have sex combs, which are groups of downhearted bristles on the uppermost joint of the forelegs. If the flies perk up up during this process, place the Petri dish back into the freezer for ten minutes or until they become immobile. 4.While sexing! , examine the phenotypes of each sound: eyes, wings, bristles and antennae. 5.Obtain eight flies (4 males, 4 females), and place them in the vial. 6.During the inaugural week, periodically watch the flies and journal either observations. register the mating behavior, mutations, and life cycle of the flies. (Steps one through half a dozen were completed by Mrs. DeYoung; details were provided in the packet). 7.When the F2 flies have emerged,...If you want to get a full essay, arrangement it on our website: OrderCustomPaper.com

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